DNA Replication and Proteins That Bind to DNA
نویسندگان
چکیده
منابع مشابه
Coupled dynamics of DNA breathing and of proteins that selectively bind to single-stranded DNA.
We study the size fluctuations of a local denaturation zone in a DNA molecule in the presence of proteins that selectively bind to single-stranded DNA, based on a (2+1)-dimensional master equation. By tuning the physical parameters we can drive the system from undisturbed bubble fluctuations to full, binding-protein-induced denaturation. We determine the effective free-energy landscape of the D...
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Recent protein engineering efforts have generated artificial transcription factors that bind new target DNA sequences and enzymes that modify DNA at new target sites. Zinc-finger-based transcription factors are favored targets for design; important technological advances in their construction and numerous biotechnological applications have been reported. Other notable advances include the gener...
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Continuous labelling of callus with H-thymidine results in intermittent peaks of H-DNA per chloroplast, showing synchrony of division. The increase in H-DNA could be due to several replication rounds, and the drop to successive plastid divisions without intervening DNA synthesis. The level of DNA-binding proteins in the chloroplast parallels the peaks of plastidal DNA synthesis; such pro...
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The MCM proteins identify a group of ten conserved factors functioning in the replication of the genomes of archae and eukaryotic organisms. Among these, MCM2-7 proteins are related to each other and form a family of DNA helicases implicated at the initiation step of DNA synthesis. Recently this family expanded by the identification of two additional members that appear to be present only in mu...
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Nuclear factor I is a 47,000-dalton protein isolated from human HeLa cells that is required for the in vitro replication of adenovirus DNA. This protein was previously shown to bind specifically to nucleotides 17-48 of the left-hand terminus of cloned adenovirus serotype 5 DNA. An in vitro assay for DNA sequences that compete with adenovirus DNA for the binding of nuclear factor I has been deve...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1971
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.68.10.2549